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| Title: | DYNAMICS OF GAS PHASE OLIGONUCLEOTIDES | |
| DOI No: | 10.1142/9789812701879_0046 | |
| Source: | CLUSTERS AND NANO-ASSEMBLIES (pp 393-406) | |
| Author(s): | ALLISON S. DANELL
Rowland Institute at Harvard, 100 Edwin H. Land Boulevard, Cambridge, MA 02142, USA RYAN M. DANELL Rowland Institute at Harvard, 100 Edwin H. Land Boulevard, Cambridge, MA 02142, USA JOEL H. PARKS Rowland Institute at Harvard, 100 Edwin H. Land Boulevard, Cambridge, MA 02142, USA |
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| Abstract: | The dynamics of biomolecule conformations in gas phase have been studied by applying methods which measure laser-induced fluorescence from trapped ions. The fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. The degree of end terminal fraying observed for 14-mer duplexes is consistent with gas phase calculations. During these studies, the autodetachment of electrons from double- and single-stranded oligonucleotide anions was observed. This process is not observed to occur for oligonucleotides in solution phase and it is important to understand how it impacts the interpretation of gas phase dynamics. A strong dependence of the autodetachment rate on base composition has been identified. FRET measurements were performed as a function of charge state to measure the relative extension of the single strands as a function of charge state and were consistent with preliminary calculations of the single strand conformation. Finally, the redesigned optical apparatus and CW laser excitation will be discussed. The capability to obtain fluorescence data from a single trapped ion will be demonstrated. | |
| Full Text: | View full text in PDF format (584KB) | |
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