BIOLUMINESCENCE AND CHEMILUMINESCENCE

BIOLUMINESCENCE AND CHEMILUMINESCENCE
Proceedings of the 11th International Symposium

Monterey, California, USA, 6 - 10 September 2000

CONTENTS


FRONT MATTER	i
INTRODUCTION TO THE 11^TH SYMPOSIUM James F. Case and Bruce H. Robison	1
IN MEMORIAM
pH 14 SCIENCE: McELROY AND THE PURSUIT OF FUNDAMENTAL RESEARCH J. WOODLAND HASTINGS	5
PART 1. MARINE BIOLUMINESCENCE
HETERODIMERIZATION BETWEEN BLUE AND GREEN FORMS OF AEQUOREA VICTORIA GFP C. M. BARBIERI, C. M. THOMSON and W. W. WARD	13
THE BIOLUMINESCENT BAYS OF VIEQUES - A MESSAGE TO THE SCIENTIFIC COMMUNITY BARBARA BERNACHE-BAKER	17
INTRACELLULAR IMAGING OF BIOLUMINESCENCE IN PYROCYSTIS NOCTILUCA GUDRUN BEHRMANN, R. HARDELAND, W. STICKAN and INA SIEBERT	19
WEAK ELF MAGNETIC FIELD EFFECTS ON Gonyaulax sp. BIOLUMINESCENCE MAJA BERDEN, ALEXIS ZRIMEC and IGOR JERMAN	23
DIEL VARIATIONS OF IN SITU PLANKTONIC BIOLUMINESCENCE A.S. CUSSATLEGRAS and P. GEISTDOERFER	27
ON THE FUNCTION AND EVOLUTION OF BACTERIAL LUMINESCENCE AGATA CZYZ and GRZEGORZ WEGRZYN	31
BIOLUMINESCENCE IN THE BRITTLE STAR AMPHIPHOLIS SQUAMATA (ECHINODERMATA): AN OVERVIEW OF 10 YEARS OF RESEARCH DIMITRI D. DEHEYN	35
INVOLVEMENT OF CALCIUM IN THE LUMINESCENCE CONTROL OF THREE OPHIUROID SPECIES (ECHINODERMATA): A COMPARATIVE STUDY YANNICK DEWAEL and JEROME MALLEFET	39
BIOLUMINESCENCE AND GENETIC VARIABILITY IN AMPHIPHOLIS SQUAMATA S. DUPONT, J. MALLEFET and P. BARET	43
ORIGIN AND CONSEQUENCES OF AGGREGATION ON BIOLUMINESCENCE DISTRIBUTION IN THE BRITTLE STAR AMPHIPHOLIS SQUAMATA S. DUPONT, F. DUMONT and J. MALLEFET	47
EFFECT OF HABITAT ON INTRASPECIFIC DIVERSITY OF BIOLUMINESCENCE OF THE OPHIUROID AMPHIPHOLIS SQUAMATA S. DUPONT, G. VANDEMEULEBROECKE, J. MALLEFET, M.-T. COSTANZO and L. SALPIETRO	51
SENSITIVITY OF OBELIN AND APOOBELIN TO DIGESTION BY SOME PROTEASES L.A. FRANK, V.S. BONDAR and E.S. VYSOTSKI	55
BIOLUMINESCENT PATROLLING OF MARINE ECOSYSTEMS - BIOALARM J.I. GITELSON, L.A. LEVIN, A.P. SHEVIRNOGOV and R.N. YTYUSHEV	59
LUMINOUS BACTERIA CULTURE COLLECTION IBSO: NATURAL BIODIVERSITY AND APPLIED ASPECTS J.I. GITELSON, G.A. VYDRYAKOVA, A.M. KUZNETSOV, E.K. RODICHEVA, S.E. MEDVEDEVA and YU.V CHUGAEVA	63
OBSERVATIONS OF BIOLUMINESCENCE ON THE ABYSSAL SEAFLOOR P.J. HERRING, I.G. PRIEDE, P.M. BAGLEY, S. WAY and J.C. PARTRIDGE	67
LARGE SCALE BIOLUMINESCENT PHENOMENA IN THE OCEAN P.J. HERRING	71
ALL THREE CALCIUM-BINDING SITES PARTICIPATE IN THE REGULATION OF OBELIN LUMINESCENCE B.A. ILLARIONOV, V.A. ILLARIONOVA, V.S. BONDAR, E.S. VYSOTSKI and JOHN R. BLINKS	75
WHERE DOES MANGANESE BIND WHEN IT TRIGGERS THE LUMINESCENCE OF CALCIUM-REGULATED PHOTOPROTEINS? B.A. ILLARIONOV, V.A. ILLARIONOVA, V.S. BONDAR, E.S. VYSOTSKI and JOHN R. BLINKS	79
DIVERGENCE OF THE PROTEINS FOR BACTERIAL BIOLUMINESCENCE WAS DRIVEN BY GC RATIO REQUIREMENTS FOR BACTERIAL GENOMES TIMOTHY C. JOHNSTON and JAMES R. COX	83
PURIFICATION AND PROPERTIES OF THE LUCIFERASE FROM THE MARINE OSTRACOD Vargula hilgendorfii KOJI KOBAYASHI, YOSHIHIRO OHMIYA, DAISAKU SHINOHARA, YOSHIHIRO OHMIYA, TAKUJI NABETANI and HARUKI NIWA	87
ON THE ORIGIN OF BIOLUMINESCENT SYSTEMS Y. A. LABAS, M.V. MATZ and V.A. ZAKHARTCHENKO	91
FLOW-STIMULATED DINOFLAGELLATE BIOLUMINESCENCE MICHAEL I. LATZ and JIM ROHR	95
PROTEIN CONFORMATIONAL CHANGES IN OBELIN SHOWN BY ¹⁵N-HSQC NUCLEAR MAGNETIC RESONANCE JOHN LEE, JOHN N. GLUSHKA, SVETLANA V. MARKOVA and EUGENE S. VYSOTSKI	99
KINETICS AND FLUORESCENCE OF A BACTERIAL LUCIFERASE MUTANT WITH ONLY TWO TRYPTOPHANS LEO LIN, ROSE SZITTNER and EDWARD MEIGHEN	103
NATURAL ANIMAL COLORATION CAN BE DETERMINED BY A NON-FLUORESCENT GFP HOMOLOG KONSTANTIN A. LUKYANOV, ARKADY F. FRADKOV, NADYA G. GURSKAYA, MIKHAIL V. MATZ, YULII A. LABAS, ALEKSANDR P. SAVITSKY, XIAONING ZHAO, YU FANG, WENYAN TAN and SERGEY A. LUKYANOV	107
MEASUREMENT OF ACYL HOMOSERINE LACTONES WITH MASS SPECTROMETRY JOHN MAKEMSON and ANATOL EBERHARD	111
OBELIN HYPEREXPRESSION IN E. coli, PURIFICATION AND CHARACTERIZATION SVETLANA V. MARKOVA, EUGENE S. VYSOTSKI and JOHN LEE	115
MULTIPLE REGULATORY PROTEINS CONTROL INDUCTION OF LUMINESCENCE IN VIBRIO HARVEYI CAROL MIYAMOTO, JAI CHATTERJEE, YIHSING LIN and EDWARD MEIGHEN	119
SPATIAL AND TEMPORAL VARIABILITY OF BIOLUMINESCENCE POTENTIAL IN COASTAL REGIONS MARK A. MOLINE, ERIKA K. HEINE, JAMES F. CASE, CHRISTEN M. HERREN and OSCAR SCHOFIELD	123
SYNTHESES OF THE MECHANISM-BASED INHIBITORS OF COELENTERAZINE BIOLUMINESCENCE H. NAKAMURA, C. WU, M. KATO, M. KIMURA and S. INOUYE	127
CHIMERIC BACTERIAL LUCIFERASES ROSE SZITTNER, LEO LIN and EDWARD MEIGHEN	131
OBELIN CRYSTAL STRUCTURE: IMPLICATIONS FOR THE BIOLUMINESCENCE MECHANISM EUGENE S. VYSOTSKI, ZHI-JIE LIU, LU DENG, JOHN ROSE, B.C. WANG and JOHN LEE	135
EFFICIENCY OF APOPHOTOPROTEIN CHARGING DEPENDS STRONGLY ON PROTEIN CONCENTRATION E.S. VYSOTSKI, L.A. FRANK and V.S. BONDAR	139
PART 2. FIREFLY BIOLUMINESCENCE
MUTATIONAL STUDIES OF STRINGENTLY CONSERVED FIREFLY LUCIFERASE ACTIVE SITE RESIDUES BR BRANCHINI, RA MAGYAR, MH MURTIASHAW, NC PORTIER and M ZIMMER	145
CENTRAL CONTROL OF LARVAL FIREFLY LUMINESCENCE ALBERT D. CARLSON	149
A NEUROBEHAVIORAL APPROACH TO FIREFLY FLASH CODE EVOLUTION JAMES F. CASE and JOHN BUCK	153
MECHANISMS OF SYNCHRONY IN A COASTAL GEORGIA FIREFLY JONATHAN COPELAND, KELSIE FITZGERALD and ANDREW MOISEFF	157
STABILIZATION OF FIREFLY LUCIFERASE ACTIVITY AGAINST OXIDATION WITH ANTIOXIDANTS R.H. DAVIES, R.A. VAN WAGENEN and J.D. ANDRADE	161
GUANIDINIUM CHLORIDE-INDUCED INACTIVATION OF FIREFLY LUCIFERASE AND ITS REACTIVATION E. I. DEMENTIEVA, L. G. MALOSHENOK and N. N. UGAROVA	165
LUCIFERASE KNOCK – OUT MUTANTS BY SITE DIRECTED MUTAGENESIS OF THE AMP BINDING SITE GABRIELA ISPAS, I. FAMELAER, FATIMA ELMALKI and M. JACOBS	169
SPECTRAL TUNING OF BIOLUMINESCENCE EMISSION AND VISUAL SENSITIVITY AMONG BEETLES ABNER B. LALL, ALEXANDRE A. CARVALHO, JOHN M. de SOUZA, ETELVINO J. H. BECHARA and THOMAS W. CRONIN	173
MATING BEHAVIOR OF A SYNCHRONOUS NORTH AMERICAN FIREFLY A. MOISEFF, J. COPELAND, M. F. KUBKE and L. FAUST	177
PROTEIN ENGINEERING AND MOLECULAR MODELLING OF FIREFLY LUCIFERASE SE PREBBLE, RL PRICE, B LINGARD, LC TISI and PJ WHITE	181
LUCIFERASE IMAGING REVEALS DISTINCT PATTERNS OF GENE REGULATION IN LIVE BRAIN SLICES LAURA A SIGWORTH, TIFFANY R. CHANDLER, LUJIAN LIAO and MICHAEL E. GEUSZ	185
MUTAGENESIS OF SOLVENT-EXPOSED HYDROPHOBIC RESIDUES IN FIREFLY LUCIFERASE LAURENCE TISI, CHRISTOPHER LOWE and JAMES MURRAY	189
THERMOINACTIVATION AND REACTIVATION OF FIREFLY LUCIFERASE N. N. UGAROVA, E. I. DEMENTIEVA and I. A. LUNDOVSKIKH	193
PLAYING WITH LUCIFERIN - NEW RESULTS ON THE LUMINESCENCE OF A WELL-KNOWN MOLECULE D WEISS, R BECKERT, K LAMM, WJ BAADER, EJH BECHARA and CV STEVANI	197
DESIGN AND SELECTION OF FIREFLY LUCIFERASES WITH NOVEL IN VIVO AND IN VITRO PROPERTIES T.L. WILLEY, D.J. SQUIRRELL and P.J. WHITE	201
PART 3. CHEMILUMINESCENCE
MICROWAVE-ASSISTED SYNTHESIS OF CHEMILUMINESCENT ACRIDINIUM SALTS MACIEJ ADAMCZYK, PHILLIP G. MATTINGLY, YON-YIH CHEN and JAMES R. FINO	207
TOO MUCH OF A GOOD THING? MODULATING THE SIGNAL OF ACRIDINIUM-9-CARBOXAMIDE SALTS MACIEJ ADAMCZYK, YON-YIH CHEN, JEFFREY R. FISHPAUGH, PHILLIP G. MATTINGLY, JEFFREY A. MOORE, YOU PAN, KEVIN SHREDER and ZHIGUANG YU	211
RECENT ADVANCES IN CHEMILUMINESCENT ENZYME SUBSTRATES H. AKHAVAN-TAFTI, R. DE SILVA, K. THAKUR, R. A. EICKHOLT, C. CHROVIAN, K. LAUWERS, R. S. HANDLEY, S. SIRIPURAPU, C. ENDRESZL and A. P. SCHAAP	215
ANTIBACTERIAL AND ANTIOXIDANT LEAF FRACTIONS FROM ACTINIDIA CHINENSIS A BASILE, S SORBO, ML VUOTTO, J SEPE, R MIRANDA, MTL IELPO, I SIMEONE, M MAESTRALE, L RICCIARDI, P DE SOLE, L FERRARA and D MONTESANO	219
ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES IN FEIJOA SELLOWIANA SKIN AND PULP A BASILE, ML VUOTTO, L RICCIARDI, L PEZONE, D MANCINO, F SENATORE and R DE PRISCO	223
EFFECT OF SOLVENTS AND POLYMERS ON THE BORONIC ACID ENHANCED PEROXIDASE - LUMINOL - PEROXIDE REACTION GIOVANNI MAGLIA and LARRY J KRICKA	227
A NEW VISIBLE OSCILLATING CHEMILUMINESCENCE SYSTEM WITH TETRAALKYLAMMONIUM HYDROXIDE A. SAMADI-MAYBODI and S.M. OURAD	231
AN ALTERNATIVE TO CHEMILUMINESCENT DETECTION: A FLUOROMETRIC METHOD TO QUANTITATE ACRIDINIUM IN ACRIDINIUM LABELED PROTEINS DINESH O. SHAH and VINCE A. SALBILLA	235
DECOMPOSITION OF "ENVIRONMENTAL HORMONES" BY SINGLET OXYGEN NOBUTAKA SUZUKI, IWAO MIZUMOTO, TAKESHI NAGAI, TATEO NOMOTO, HIROKO MATSUYA, BINKOH YODA, KENYA KOZAWA and AKIYA KOZAWA	239
CHEMILUMINESCENCE AS A PRODUCT OF THE SPONTANEOUS OXIDATION OF SULFIDE IN SEAWATER DAVID W. TAPLEY	243
A METHOD FOR HIGHLY EFFICIENT CHEMILUMINESCENCE OF IMIDAZOPYRAZINONE IN WATER K. TERANISHI	247
AB INITIO CALCULATION FOR D-(-)-LUCIFERIN AND ITS INTERMEDIATES IN DIMETHYL SULFOXIDE NAOHISA WADA and KEIICHIRO SAMESHIMA	251
PHENOLIC AND INDOLIC RADICAL SCAVENGERS AS LUMINOPHORES JANINE WENDLER, SABINE HOLST, R. HARDELAND, BEATA K. ZSIZSIK, CHANDANA HALDAR and B. POEGGELER	255
PART 4. APPLICATIONS OF BIOLUMINESCENCE
NEW BIOLUMINESCENT ASSAY OF ALKALINE PHOSPHATASE USING ADENOSINE-3'-PHOSPHATE-5'-PHOSPHOSULFATE AS SUBSTRATE AND LUCIFERIN LUCIFERASE REACTION H. ARAKAWA, M. SHIOKAWA, O. IMAMURA, A. KOKADO and M. MAEDA	261
BIOLUMINESCENCE AS A TOOL TO ASSIST IN THE DEVELOPMENT OF BIOSORBENTS FOR FOOD-BORNE BACTERIAL PATHOGENS L. BROVKO, W. SUN, F. KHOSRAVI and M. GRIFFITHS	265
BIOSENSORS FOR THE DETECTION OF HEAVY METALS AND GENOTOXICITY P. CORBISIER, S. TAGHAVI, A. BOSSUS, E. BRITS, D. VAN DER LELIE and L. VERSCHAEVE	269
DEVELOPMENT OF AN ATP-BASED CHEMOSENSITIVITY ASSAY I. A. CREE, C.M. KURBACHER, C. SARTORI and P.E. ANDREOTTI	273
BIOLUMINESCENT METHOD TO CONTROL BACTERICIDAL EFFECT OF BLOOD V.G. FROUNDJIAN, N.N. UGAROVA and L. BROVKO	277
RAPID DETECTION OF STAPHYLOCOCCUS AUREUS AND COLIFORMS USING FIREFLY LUCIFERASE S. FUKUDA, I. MASUDA-NISHIMURA, H. TATSUMI, H. IGARASHI and S. IGIMI	281
RECOMBINANT FIREFLY LUCIFERASES AND THEIR APPLICATION FOR THE DETECTION OF MICROORGANISMS S. FUKUDA, S. MURAKAMI and H. TATSUMI	285
EVOLUTION OF A THERMOSTABLE LUCIFERASE FOR APPLICATION IN ATP ASSAYS R.R. HANNAH, M.P. HALL, M.T. BECK and K.V. WOOD	289
DETECTION OF SURFACE ANTIGENS ON TUMOR CELLS BY IMMUNOLUMINESCENCE AND ENZYMATIC ANALYSIS FRIEDEMANN LAUBE	293
SOLUBILITY OF A LUCIFERASE-GFP FUSION PROTEIN IN BACTERIAL AND MAMMALIAN CELLS G. M. NELSON and A. ESCHER	297
RAPID DETECTION OF FOOD-BORNE BACTERIA USING BACTERIOPHAGE AND AK BIOLUMINESCENCE RL PRICE, MJ MURPHY, DJ SQUIRRELL and MF SANDERS	301
BIOLUMINESCENT ASSAY OF ATP LEAKAGE FROM YEAST CELLS AFTER PHOTODYNAMIC DAMAGE N. A. ROMANOVA, EV. V. POMETUN, L. YU. BROVKO, N. N. UGAROVA and A. P. SAVITSKY	305
OPTIMIZING BIOSENSOR DESIGN WITH COMPUTER MODELING: A CASE STUDY INVOLVING CREATINE J.W. SMITH, R.H. DAVIES, J.D. ANDRADE and R.A. VAN WAGENEN	309
BIOMATE: DEVELOPMENT OF CUSTOM-DESIGNED BIOLUMINESCENT SENSORS FOR TOXICITY TESTING SIOUXSIE WILES, ANDREW WHITELEY, JIM PHILP and MARK BAILEY	313
PART 5. APPLICATIONS OF CHEMILUMINESCENCE
CHEMILUMINESCENT ASSAY OF ALKALINE PHOSPHATASE USING DIHYDROXYACETONE PHOSPHATE AS SUBSTRATE WITH LUCIGENIN AMANE KOKADO, HIDETOSHI ARAKAWA and MASAKO MAEDA	319
RESPONSE OF AN ENHANCED CHEMILUMINESCENCE ASSAY TO SELECTED SUBSTANCES JAMES C. MC HILLIS, B. RIPPEY and S. P. FITZGERALD	323
A CHEMILUMINESCENT WHOLE CELL BIOSENSOR FOR ASSESSING ESTROGENIC ACTIVITY P. PASINI, G. GENTILOMI, M. GUARDIGLI, M. BARALDINI, M. MUSIANI and A. RODA	327
PART 6. IMMUNOASSAY AND DNA PROBE ASSAYS
CARBONYL-REACTIVE CHEMILUMINESCENT LABELS: ACRIDINIUM HYDROXYLAMINES (AHA) MACIEJ ADAMCZYK, JONATHAN GROTE, PHILLIP G. MATTINGLY, JEFFREY A. MOORE and YOU PAN	333
THIOL-SPECIFIC ACRIDINIUM REAGENTS FOR LABELING PROTEINS AND NUCLEIC ACIDS MACIEJ ADAMCZYK, PHILLIP G. MATTINGLY, JEFFREY A. MOORE and SUSHIL D. REGE	337
QUANTITATION OF FREE CHEMILUMINESCENT ACRIDINIUM-9-CARBOXAMIDE SALTS IN BIOCONJUGATES JOHN C. GEBLER, MACIEJ ADAMCZYK, KEVIN SHREDER and JIANG WU	341
CHARACTERIZATION OF ACRIDINIUM-9-CARBOXAMIDE—MONOCLONAL ANTIBODY BIOCONJUGATES BY ELECTROSPRAY IONIZATION MASS SPECTROMETRY JOHN C. GEBLER, MACIEJ ADAMCZYK, KEVIN SHREDER and JIANG WU	345
CHEMILUMINESCENT IMMUNOASSAYS FOR THE DETECTION OF ORGANOCHLORINE PESTICIDES S. GIROTTI, F. FINI, P. RAUCH, B. MICKOVA, L. KARASOVA, L. FUKAL, A. ABAD, J. J. MANCLUS, J. V. MERCADER and A. MONTOYA	349
DEVELOPMENT OF BIOLUMINESCENT ASSAY FOR PYRUVATE PHOSPHATE DIKINASE AND ITS APPLICATION TO BIOLUMINESCENT ENZYME IMMUNOASSAY KATSUTOSHI ITO, KAZUMI NISHIMURA, SEIJI MURAKAMI, HIDETOSHI ARAKAWA and MASAKO MAEDA	353
DEVELOPMENT AND APPLICATION OF A BIOLUMINESCENT IMMUNOASSAY USING AEQUORIN AS A LABEL M. MIRASOLI, S. DEO, J.C. LEWIS and S. DAUNERT	357
RAPID BACTERIAL DETECTION USING ADENYLATE KINASE (AK) IN A MAGNETIC BEAD IMMUNOASSAY NV SELLICK-HARMON, MJ MURPHY, RL PRICE and DJ SQUIRRELL	361
COMBINED HIV ANTIGEN AND ANTIBODY ASSAY ON A FULLY AUTOMATED CHEMILUMINESCENCE BASED ANALYZER DINESH SHAH, CHI-DEU CHANG, KEVIN CHENG, ANDREA WACHTER and JAMES STEWART	365
LUMINESCENT PCR-ELISA ASSAY TO DETECT TB XIAOLIN YANG	369
THE QUANTIFICATION OF REACTIVE OXYGEN SPECIES AND THEIR SCAVENGER FROM PHOTON EMISSION Y. YOSHIKI, Y. AKIYAMA, K. ABE and K. OKUBO	373
DEVELOPMENT OF A CHEMILUMINESCENCE IMMUNOASSAY FOR CLARA CELL PROTEIN AT SUB-PM LEVELS GIJSBERT ZOMER	377
PART 7. CELLULAR LUMINESCENCE
MOLECULAR OXYGEN, PHAGOCYTE MICROBICIDAL ACTION AND LUMINESCENCE ROBERT C. ALLEN	383
THE CHEMIEXCITATION OF THE PHOTOSENSITIZERS IN THE CELLULAR CULTURES MARTINA BANCIROVA, HANA KOLAROVA, RENE LENOBEL and JAN LASOVSKY	387
EFFECT OF METHOTREXATE ON LEUCOCYTE RESPIRATORY BURST IN RHEUMATOID ARTHRITIS PATIENTS P. DE SOLE, G. NOCCA, R. FRESU, A. POMPA and A. ZOLI	391
CRITICAL LEG ISCHAEMIA: IN VITRO MODULATION OF PERIPHERAL LEUCOCYTE ACTIVITY BY (+)-CATECHIN P DE SOLE, ML VUOTTO, R MIRANDA, C NAPPO, MTL IELPO, V MOSCATIELLO, E LAGHI, G ASSO, L RICCIARDI and A BASILE	395
THE FUNCTION OF NEUTROPHIL GRANULOCYTES AFTER RADIOTHERAPY P. HENGSTER, G. BRANDACHER, S. SCHNEEBERGER and A. DE VRIES	399
IMPAIRED GRANULOCYTE FUNCTION PRECEDES INFECTION IN IMMUNO-COMPROMISED ALLOGRAFT RECIPIENTS PAUL HENGSTER, WALTER MARK, RAIMUND MARGREITER and GERD EGGER	403
INHIBITORY EFFECT OF TERBINAFINE ON REACTIVE OXYGEN SPECIES (ROS) GENERATION BY CANDIDA ALBICANS U.-CH. HIPLER, CH. SCHROETER, B. HIPLER and U. WOLLINA	407
FLUORESCENCE ANALYSIS OF REACTIVE OXYGEN SPECIES (ROS) GENERATED BY SIX ISOLATES OF ASPERGILLUS FUMIGATUS U-CH. HIPLER, U. WOLLINA, D. DENNING and B. HIPLER	411
ANALYSIS OF NEUTROPHIL-DERIVED REACTIVE OXYGEN SPECIES USING A HIGHLY SENSITIVE CHEMILUMINESCENCE PROBE L012 I. IMADA, E. F. SATO, Y. KIRA, Y. MINAMIYAMA, R. KONAKA, Y. ICHIMORI, S. HONDA and M. INOUE	415
NADPH OXIDASE ACTIVITY CHANGES BY ATHEROGENIC AND ATHEROPROTECTIVE LIPOPROTEINS S. KOPPRASCH, J. GRAESSLER, H.E. SCHRÖDER and J. PIETZSCH	419
TRAINING EFFECTS ON CHEMILUMINESCENT RESPONSE OF ALVEOLAR MACROPHAGE IN MATURED RATS T. KUMAE and H. ARAKAWA	423
INHIBITION ANALYSIS OF CHICKEN HETEROPHIL LUMINOL AND LUCIGENIN LUMINESCENCE GERALD A. MERRILL and ROBERT C. ALLEN	427
NEUTROPHIL CL ACTIVITY IN A HYPER-IgE ASSOCIATED LEUKOCYTOCLASTIC VASCULITIS CASE ML VUOTTO, R MIRANDA, MTL IELPO, C NAPPO, D MONTANARO, E LAGHI, L RICCIARDI, A GATTONI, C ROMANO and A CECERE	430
PHAGOCYTE ACTIVITY IN OLD AND YOUNG MICE UNDERGOING SURGICAL STRESS ML VUOTTO, MTL IELPO, R MIRANDA, C NAPPO, D SPATUZZI, L RICCIARDI, P DE SOLE, C ASTARITA, D GARGANO and F MANGUSO	434
PART 8. REPORTER GENES IN CELL BIOLOGY AND ANALYSIS
SECRETED RENILLA LUCIFERASE AS A MARKER OF GENE EXPRESSION IN MAMMALIAN SYSTEMS M. FILIPPOVA, J. LIU and A. ESCHER	441
THE GLIAL CELL-LINE DERIVED NEUROTROPHIC FACTOR-LUCIFERASE FUSION IS FULLY FUNCTIONAL UPON SECRETION FROM MAMMALIAN CELLS H. LIU and A. A. SZALAY	445
VISUALIZATION OF BACTERIA IN LIVE ANIMALS USING LUCIFERASE LABELING SHAHROKH SHABAHANG and ALADAR A. SZALAY	449
GFP IN PLANT BIOTECHNOLOGY AND AGRICULTURE C. NEAL STEWART, JR., MATTHEW D. HALFHILL and HAROLD A RICHARDS	453
VISUALIZATION OF VACCINIA VIRUS INFECTION USING THE RENILLA-LUCIFERASE-GFP FUSION PROTEIN TATYANA M. TIMIRYASOVA, YONG A. YU, SHAHROKH SHABAHANG, ISTVAN FODOR and ALADAR A. SZALAY	457
GENOME-WIDE EXPRESSION PROFILING WITH luxCDABE GENE FUSIONS TINA K. VAN DYK, GREGORY E. GONYE, MARY JANE G. REEVE, ELLEN J. DEROSE, MAUREEN DOLAN, MICHAEL K. HANAFEY, YAN WEI, J. ANTONI RAFALSKI and ROBERT A. LAROSSA	461
INDUCIBLE GENE EXPRESSION IN VIVO USING A RENILLA LUCIFERASE – GFP FUSION CONSTRUCT YONG A. YU, SHELLEY CALTHARP and ALADAR A. SZALAY	465
SYNTHETIC LUCIFERASE GENES AS BETTER REPORTER MOLECULES YAO ZHUANG, AILEEN P. PAGUIO, MONIKA G. GRUBER and KEITH V. WOOD	469
PART 9. LUMINESCENCE IN SCIENCE AND EDUCATION
PUBLIC UNDERSTANDING OF SCIENCE AND HEALTH: THE FLORENCE PROJECT P. PINZANI, C. ORLANDO, B. SALVADORI, M. PAZZAGLI, F. TORRICELLI and L. MERCATI	475
GFP AS AN EDUCATIONAL TOOL IN BIOTECHNOLOGY DISTANCE LEARNING W. W. WARD, G.C. SWIATEK and C. M. THOMSON	479
PART 10. INSTRUMENTATION AND IMAGING OF LUMINESCENCE
THE NORTHSTAR™ HTS WORKSTATION LUMINESCENCE DETECTION SYSTEM CORINNE E. M. OLESEN, DEBORAH M. BOLDT-HOULE, SUSAN YAN, BRIAN J. D'EON, BETTY LIU, BONNIE TILLOTSON, JOHN C. VOYTA and IRENA BRONSTEIN	485
INSTRUMENTATION FOR IN VIVO IMAGING OF BIOLUMINESCENT REPORTERS B.W. RICE, M. CABLE, B. NELSON and D. STEARNS	489
DEVELOPMENT OF A TIME-RESOLVED FLUORESCENCE MICROSCOPE FOR IMAGING ANALYSIS A. RODA, M. GUARDIGLI, P. PASINI and M. BARALDINI	493
THE STUDY OF LUMINESCENCE RESONANCE ENERGY TRANSFER IN CELL EXTRACTS OF TRANSFORMED CELLS USING A LOW LIGHT IMAGING SYSTEM YUBAO WANG and ALADAR A. SZALAY	497
BACK MATTER	501
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